Erythrocyte Osmotic Fragility and Malondialdehyde Concentrations of Water Truck Pushers in Samaru, Sabon-Gari Local Government Area of Kaduna State, Nigeria

Author(s) Name

D. Usman, A. Jimoh, N. M. Gidado, J.B Balogun, Y. Tanko, A. Ahmed, A. Mohammed & J. O. Ayo

Abstract
Water vending is a significantly lucrative business laden with stressful and strenuous activities making it to be implicated in oxidative damage, lipid peroxidation and the susceptibility of erythrocytes to haemolysis. The study was designed to investigate the degree of erythrocyte osmotic fragility (EOF) and Malondialdehyde (MDA) concentrations among water vendors and non-water vendors. Copies of questionnaire were randomly distributed in the study area to a total of 192 subjects of which water truck pushers (experimental group) were 96 and non-water truck pusher (Control group) were also 96 individuals. 5ml of blood was obtained from the median cubital vein via venepuncture. Ethical clearance was obtained from the health research ethics committee of A.B.U, Zaria, Nigeria. 3ml of samples stored in EDTA bottles were used for EOF whereas serum samples obtained from 2ml of centrifuged blood were assayed biochemically for MDA concentration using TBARS assay. Data obtained were analysed using independent sample T-test and cross Tab for descriptive statistics. The result showed a significant (P < 0.05) increase in serum MDA concentrations of the experimental group as compared to controls with a value of 230.33 ± 4.75 compared to 215.61 ± 3.59nmols/mL respectively. EOF of water vendors showed significant (P < 0.05) increase with a value of 85.83 ± 0.37% when compared to 78.69 ± 0.53% for non-water truck pushers at 0.4% concentration of NaCl solution. These findings suggest that a significant increase exists in the EOF and MDA concentration of water vendors and as such the need to mitigate this effect.

Keywords

Water vendors, Oxidative stress, Lipid peroxidation, Erythrocyte osmotic fragility, Ergonomics.

Please cite this article as

D. Usman, A. Jimoh, N. M. Gidado, J.B Balogun, Y. Tanko, A. Ahmed, A. Mohammed & J. O. Ayo (2019). Erythrocyte Osmotic Fragility and Malondialdehyde Concentrations of Water Truck Pushers in Samaru, Sabon-Gari Local Government Area of Kaduna State, Nigeria. International Journal of Recent Research and Applied Studies, 6, 8(2), 7-17.