Isolation, Screening and Optimization of Phosphate Solubilizing Bacteria and it Effective Role on Plant Growth Promotion

Abstract:

In this present study, phosphate solubilizing bacterial (PSB) strain was isolated from Rhizosphere beans plant soil sample from Srikalahasthi A.P, India and used for screening and production alkaline phosphatase in Luria broth medium. The parameters like Time, Temperature, pH, Carbon sources and Nitrogen sources were optimized for better production of alkaline phosphatase. In consideration of time parameter at 48 hrs incubation of specific strain showed maximum alkaline phosphatse production. Among different temperatures the maximum alkaline phosphatse was recorded at 37 °C temperature. In pH parameter, pH 8 showed maximum production of alkaline phosphatase activity in isolated species. In the case of carbon source lactose and in nitrogen source Ammonium nitrate were showed the higher production of alkaline phosphatase. The partial purification of alkaline phosphatase was employed by ammonium sulphate precipitation method, dialyzed and characterized. The protein profile was analyzed in SDS-PAGE was carried out. The phosphatase enzyme helps the growth of Maize plant.

Author Name : Anuradha Thallapaka

Keywords: Phosphatse enzyme, Carbon source, SDS-PAGE, Maize plant.