Microbial Protease: A Degumming Agent

Abstract :

Degumming with soap and soda ash for 20 min at boil gives a weight loss of 24%, whereas degumming with
enzyme at 50°C for 2 h gives a weight loss of about 26-28%. Treatment with proteolytic enzyme requires much lower
temperature than that in the conventional process. Processing of silk with enzyme under the above condition is likely to retain the luster and softness of silk. To economize the production of Proteolytic enzyme, the conditions like temperature, time, pH have been optimized. The efficiency of this enzyme has been studied in terms of weight loss, whiteness index, dyed and assessed for color value, texture feel and luster.

Highlights

  • Eco friendly Degumming was done with microbes.
  • Penicillium citrinum and Bacillus subtilis was producing proteolytic enzyme.
  • Degumming of silk yarn was best done on temperature 50˚C and pH 9 for 2 hours.
  • Colour measurement was done.

Keywords: Degumming, Proteolytic enzyme, Silk, Penicillium citrinum, Bacillus subtilis Abbreviations: NA – Nutrient Agar, PDA – Potato Dextrose Agar, P.citrinum- Penicillium citrinum, B.subtilis-Bacillus subtilis


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