PREPARATION OF PROTEIN NANOFIBER FOR WOUND HEALING APPLICATION

Author Name(s):

C.Narayanan¹,A.K Nivedha²,S.Krishna priya³

¹ Professor ,^2,3. Assistant Professors,

Dhanalakshmi Srinivasan Engineering College

 Abstract

Keratin is one of the most copious protein which can be used in a variety of biological applications due to its physical and mechanical properties. The focus here is on keratin, one of the most unexploited non-food natural protein used for tissue engineering and for the production of affinity based Nanofibers because of its biodegradability and biocompatibility. Extraction of keratin from human hair waste using Shindai method and quantifying the protein using SDS-PAGE. Transforming keratin, gentamycin(antibiotic drug) and PVA polymer(synthetic polymer) into nanofibers using electrospinning techniques which combines the physical and mechanical properties of keratin with parameters such as high surface to volume ratio ,three dimensional features and high porosity for nanostructured nonwoven fibers. The electrospun nanofibers will analyze by scanning electron microscopy (SEM) and Transmission Electron microscopy (TEM) for characterizing its morphology and structure. These qualities will develop the keratin based nanofibers for tissue engineering applications such as wound healing and inflammatory responses.   

Keratin is one of the most copious protein which can be used in a variety of biological applications due to its physical and mechanical properties. The focus here is on keratin, one of the most unexploited non-food natural protein used for tissue engineering and for the production of affinity based Nanofibers because of its biodegradability and biocompatibility. Extraction of keratin from human hair waste using Shindai method and quantifying the protein using SDS-PAGE. Transforming keratin, gentamycin(antibiotic drug) and PVA polymer(synthetic polymer) into nanofibers using electrospinning techniques which combines the physical and mechanical properties of keratin with parameters such as high surface to volume ratio ,three dimensional features and high porosity for nanostructured nonwoven fibers. The electrospun nanofibers will analyze by scanning electron microscopy (SEM) and Transmission Electron microscopy (TEM) for characterizing its morphology and structure. These qualities will develop the keratin based nanofibers for tissue engineering applications such as wound healing and inflammatory responses.   

Keywords: Hair keratin, Shindai method, Tissue engineering, electrospun nanofibers

Please cite this article as C.Narayanan,A.K Nivedha and S.Krishna Priya  (2021)  PREPARATION OF PROTEIN NANOFIBER FOR WOUND HEALING APPLICATION . International Journal of Recent Research and Applied Studies, 8- 6(2), 6-9