Abstract :
In this study, the water and soil sample were collected from the four different oil contaminated regions of Tiruchirappalli city during premonsoon and monsoon season 2015. In which, a total of sixty (60) bacterial strains were isolated and challenged against chromium metal (Potassium chromate) solutions with four different concentrations (10 mM, 50 mM, 100 mM and 250 mM) for metal resistant studies by plate diffusion and tube dilution methods. A growth rate between 90-100% was observed for 86.5 % of the bacterial populations at 10 mM of Cr, whereas no population was growing at a growth rate of 0–80 % with 10 mM of Cr. At 50 mM of Cr, 53.5 % of the populations showed a growth rate of 81-90 % while 10 % of the population showed a growth rate of 0-80 %. A growth rate between 51-60 % was observed for 1.5 % of the bacterial populations at 100 mM of Cr, whereas no population was growing at a growth rate of 0–50 % with 100 mM of Cr. At 250 mM of Cr, 33.5 % (20 isolates) of the populations showed a growth rate of 71-80 % whereas 38.5 % of the populations showed a growth rate of 61-70 % in 250 mM concentrations. Interestingly, 1.5 % of the strains were survive in the 250 mM concentration and most of the strains were resistant to minimum level concentration (10 mM) of chromium metal. Our results show that Tiruchirappalli oil contaminated regions has a significant proportion of heavy metals and metal resistant bacteria, and these bacteria constitute a potential risk for public health.
Author Name : C. Vinothini & R. Ravikumar
Keywords: Oil contamination, Metal resistant bacteria, Copper, Chromium, Tiruchirappalli.
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