Abstract :
The yeast mediated synthesis and characterization of gold nanoparticles (AuNPs) were prepared and it also challenged against certain bacterial and fungal strains. In this present study, biosynthesized AuNPs were confirmed by analyzing the excitation due to the applied electromagnetic field of surface plasmon resonance (SPR) using UV–vis spectrophotometer at 540 nm and the peak was observed between 500-600 nm. The SEM absorption of the product was recorded as synthesis of nanoparticles spherical in structure of about 90 nm in diameter. The EDS revealed the presence of pure gold nanoparticles in higher percentages. DLS-zeta potential showed negative charge (-30.5) which indicated that the sample is moderately stable at room temperature. The S. cerevisiae biomass extracts, in HAuCl4 peaks were observed at recorded in the region between 4000 and 400 cm-1. They include 3434 cm-1, 2363 and 2079.16 cm-1, 1637 cm-1 which are associated OH stretching, C=C stretching, CH stretching, CH stretching respectively. In XRD, peaks were observed at 2θ of 38, 44, 65 and 77 are corresponding to the Bragg’s reflections such as (111), (200), (220) and (311). The sample was most effective against Salmonella typhimurium NCIM 2501 (B5) while smaller effect was noticed from Micrococcus luteus NCIM 2871 (B4) in the bacterial division. But in fungi, which was effective against Trichophyton rubrum MTCC 3272 (F4) whereas smaller effect was observed in Candida albicans MTCC 1637 (F1).
Author Name : T. Saranya
Keywords: Gold nanoparticles (AuNPs), Saccharomyces cerevisiae, Antimicrobial activity, Yeast mycelia.
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